| | Category | MI | L21 | A Bacterial Procedure: A Census of Gram-Positive and Gram- |
| | Negative Bacteria Fo |
| | Abstract | Purpose: To compare the bacteria from human feet and the shoes of |
| | human feet before and after deodorizing; if the deodorizer actually affects |
| | the human feet itself or the shoe. |
| | Hypothesis: The process of deodorizing will affect the human feet more |
| | than the human shoe. |
| | Introduction: Bacteria are found everywhere and could be pathogenic or |
| | beneficial. A gram-stain test is performed to identify the bacteria and the |
| | bacteria are visible and usually have two outcomes; gram-positive and |
| | gram-negative. |
| | Procedure: Make nutrient agar plates. Swab human feet and inoculate |
| | swab onto the agar plate. Swab the human show and inoculate onto the |
| | agar plate. Spray the human foot with the deordorizer and the human |
| | shoe, swab both with different swabs and inculate onto the agar plate. |
| | Let the bacteria grow over five days and record the growth of each day. |
| | Perform a gram-stain test to determine if the bacteria are gram-positive or |
| | gram-negative. |
| | Conclusion: The hypothesis for the experiment was correct; the human |
| | feet is affected more compared to the human shoe. The results show a |
| | larger difference between the deodorized human foot and the deodorized |
| | human shoe; there were a lot more gram-positive bacteria on the |
| | deodorized human foot compared to deodorized human shoe. |
| | Bibliography | Bailey, Regina. "How to Streak a Bacterial Culture." About: Biology, 16, |
| | Jan. 2008 |
| | <http://biology.about.com/c/ht/00/07/how_streak_bacterial_culture0962932 |
| | 483.html>. |
| | |
| | Mary T. Johnson, Ph.D., Mary T. "Basic Bacterial Culture and Identification." |
| | 23 Nov. 2003. 16 Jan. 2008 |
| | <http://web.indstate.edu/theme/micro/basic.html> |
| | |
| | Leung, Beatrice, and Shijun Liu. "Interpreting Plates." Science Buddies. 16 |
| | Jan. 2008 |
| | <http://sciencebuddies.org/mentoring/project_ideas/microbio_interpreting_p |
| | lates.shtml> |
| | |
| | "Anaerobic Bacteria Culture." Answers.com 16 Jan. 2008 |
| | <http://www.answers.com/topic/anaerobic-bacteria-culture>. |